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Document complete CultureBotAI project ecosystem with tool interconnections
Add comprehensive project ecosystem section showing how all 12 repositories work together, enabling users to understand tool relationships and workflows. Add 5 missing repositories (PFASCommunityAgents, CultureMech, eggnog_runner, eggnogtable, MATE-LLM, MicroGrowLinkService) with detailed descriptions and relationship mappings. Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
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index.md

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### 📊 [METPO Ontology](https://github.com/Knowledge-Graph-Hub/kg-microbe/tree/main/src/kg_microbe/transform/metpo)
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Microbial experimental and theoretical preference ontology for standardizing growth preference data.
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## How Our Tools Work Together
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CultureBotAI's projects form an integrated ecosystem built on the [kg-microbe knowledge graph](https://github.com/Knowledge-Graph-Hub/kg-microbe):
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- **Data processing pipelines** prepare chemical, genomic, and literature data
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- **AI agent systems** combine multiple data sources for intelligent predictions
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- **Specialized applications** target specific research domains (PFAS, lanthanide)
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- **Web services** provide API access to prediction models
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[Explore the complete project ecosystem →](/resources/#project-ecosystem--workflows)
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## Frequently Asked Questions

kg-microbe.md

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- **Knowledge Discovery**: Identify novel microbial relationships
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- **Data Mining**: Extract patterns from large-scale microbial datasets
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## Projects Built on KG-Microbe
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The kg-microbe knowledge graph serves as the foundation for numerous CultureBotAI projects:
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### Data Integration Projects
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- **CultureMech & MicroMediaParam** - Integrate chemical compound data with standardized identifiers
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- **assay-metadata** - Add phenotypic assay results from 99K+ BacDive strains
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- **MATE-LLM** - Extract and integrate literature-derived cultivation data
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- **eggnogtable** - Map genome functional annotations to ontology terms
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### Prediction & Analysis Tools
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- **MicroGrowAgents** - Multi-agent system using kg-microbe for evidence-based media design
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- **MicroGrowLink** - Graph transformer models trained on kg-microbe structure
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- **PFAS-AI & PFASCommunityAgents** - PFAS biodegradation using microbial relationships
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- **CMM-AI** - Lanthanide bioprocessing research leveraging metabolic data
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### Web Services
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- **MicroGrowLinkService** - RESTful API providing programmatic access to predictions
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[Explore all projects and their relationships →](/resources/#project-ecosystem--workflows)
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## Access and Usage
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### Repository

research.md

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- Novel organism property inference
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- Integration with laboratory information systems
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## Tools Supporting Our Research
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Our research goals are enabled by a suite of interconnected software tools:
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### For Cultivation of Novel Organisms
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- [MicroGrowAgents](/resources/#microgrowagents) - AI-driven media design with multi-agent reasoning
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- [MicroGrowLink](/resources/#microgrowlink) - Graph-based growth prediction using transformers
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- [MATE-LLM](/resources/#mate-llm) - Literature protocol extraction with LLMs
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### For Culture Optimization
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- [CultureMech](/resources/#culturemech) - Chemical compound processing pipeline
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- [MicroMediaParam](/resources/#micromediaparam) - Media composition analysis and mapping
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- [microbe-rules](/resources/#microbe-rules) - ML model optimization and comparison
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### For Growth Preference Prediction
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- [kg-microbe](/kg-microbe/) - Knowledge graph foundation with 864K+ validated species
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- [assay-metadata](/resources/#assay-metadata-bacdive-api-assay-metadata-extractor) - Phenotypic assay data from BacDive
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- [eggnog_runner & eggnogtable](/resources/#eggnog_runner) - Genome functional annotation
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[See all tools and workflows →](/resources/)
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## Collaborative Research
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We actively collaborate with:
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- **Academic institutions** developing novel cultivation techniques
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- **Industry partners** scaling up microbial production processes
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- **Industry partners** scaling up microbial production processes
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- **Government laboratories** studying environmental microorganisms
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- **Open source communities** building computational biology tools
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## Future Directions
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### Short-term Goals (1-2 years)
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- Release production version of kg-microbe knowledge graph
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- Deploy automated culture monitoring in partner laboratories
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- Publish comprehensive growth prediction models
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### Long-term Vision (3-5 years)
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- Achieve high success rate in novel organism cultivation
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- Enable fully automated microbial cultivation pipelines
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- Integrate CultureBotAI tools into standard laboratory workflows
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## Publications & Preprints

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