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UCSC Genome Browser Visualizations

Track data hubs can be used to visualize genomics signal tracks using the UCSC Genome Browser (for more information, please see the Raney et al., 2014 publication.

We developed a track data hub to visualize both maxATAC-derived in-silico and experimental ChIP-seq datasets in tandem with chromatin accessibility data. Data can be explored in multiple ways using this tool.

Visualization of Genomic Regions

After loading the track data hub (link here) in "My Data" > "Track Hubs" on the UCSC Genome Browser website, you can select specific genomic locations to visualize all subpopulation-resolved maxATAC predictions and chromatin accessibility information in that area. For instance, the image below shows maxATAC predictions in each of the sixteen subpopulations at the promoter and gene body of IFNG.

260201_CD4_T_Cell_UCSC_Genome_Browser_Screenshot

Visualization of Specific Subpopulations

If you would like to visualize maxATAC predictions for a subset of subpopulations (e.g., the Th1 resting and active subpopulations), navigate to the track settings page for this track and select the desired subopoulations to visualize, as shown below.

260201_CD4_T_Cell_UCSC_Genome_Browser_Settings

Once the changes are submitted, only the desired subpopulations appear in the Browser.

260201_CD4_T_Cell_UCSC_Genome_Browser_Th1_Screenshot

Identification of Specific TFBS

By right-clicking on one of the subpopulation tracks and selecting the "pack" option, you can visualize all TFs with predicted binding sites in that region for the chosen subpopulation.

260201_CD4_T_Cell_UCSC_Genome_Browser_Settings_Two

For example, the following screenshot demonstrates TFBS for all of the TFs that are predicted to bind at an IFNG promoter in the resting Th1 subpopulation.

260201_CD4_T_Cell_UCSC_Genome_Browser_Th1_Rest_Screenshot