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do not index starbam
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workflow/rules/rsem.smk

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@@ -59,7 +59,7 @@ rule get_rsem_counts:
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set -exuf -o pipefail
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cd {params.rsemdir}
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# inter strandedness
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samtools index -@{threads} {input.starbam}
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# samtools index -@{threads} {input.starbam}
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infer_experiment.py -r {input.bed12} -i {input.starbam} -s 1000000 > {output.strandinfo}
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# Get strandedness to calculate Forward Probability
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fp=$(tail -n1 {output.strandinfo} | awk '{{if($NF > 0.75) print "0.0"; else if ($NF<0.25) print "1.0"; else print "0.5";}}')

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