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6060Depending on the DNA library design, your amplicon sequences could be tagged with barcodes in different ways.
6161NextITS supports multiple multiplexing strategies that can be specified with the ` --lima_barcodetype ` parameter:
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63- - ` dual_symmetric ` - identical barcodes at both ends
63+ - ` dual_symmetric ` - identical barcodes at both ends (default)
6464- ` dual_asymmetric ` - different barcodes at 5' and 3' ends
65- - ` dual ` - a mixture of symmetric and asymmetric barcodes (default)
65+ - ` dual ` - a mixture of symmetric and asymmetric barcodes
6666- ` single ` - barcodes only at one end
6767
6868For dual-barcoding schemes, provide barcode pairs in the FASTA file using the following format:
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