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<title>piRNA InmunoPrecipitation databaseb</title>
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    <h1 class="w3-jumbo">piRNA InmunoPrecipitation database </h1>
    <p>(piRNA-IPdb v2)</p>
    <img src="images/piRNA-PIWI.png" alt="piRNA" style="border:3px solid black;" class="w3-image" width="600" height="720">
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  <!-- Database Section -->
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    <h2 class="w3-text-black">What is a piRNA</h2>
    <hr>
    <p>PIWI-interacting RNAs (<b>piRNAs</b>) are single-stranded
small non-coding RNAs initially discovered in germ cells with
a role as defenders of genomic integrity in the
germline, acting as post-transcriptional repressors of
transposable elements. With a regular range of 24-32 nucleotides, piRNAs are associated with the PIWI
clade of Argonaute family proteins (in the mouse
MIWI, MILI or MIWI2). Processed piRNAs
preferentially accommodate uridine residues at
5’-end and 2'-O-methylated 3'-end to increase
stability of these molecules. piRNAs have also been
detected in somatic cells with diverse potential
functions indicating its high plasticity and pleiotropic
activity.
    </p>

    <h2 class="w3-text-black">Database</h2>
    <hr>
    <p>
    We selected those piRBase datasets obtained by immunoprecipitation from wild-type mice. Using a
custom bioinformatic pipeline we analyze and calculate each piRNA count per million for every
dataset when these data were available. As applied in first version [1].

    </p>

        <p>
In total, 23 datasets were selected resulting in
18,904,992 unique sequences that were detected as bound
to any PIWI protein. It should be noted that the focus of
piRNA studies and sequencing has been on the germline
and in particular on the male line. The heterogeneity of
the datasets composition assures the presence of piRNAs
expressed in different moments of development (adult or
distinct embryonic stages), in different biological samples
(testes or spermatids) and bound to different PIWI
protein (MIWI, MILI or MIWI2).
    </p>

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	<h2 class="w3-padding-16 w3-text-black">Database statistics </h2>
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        <span class="w3-xlarge">23</span><br>
        Full datasets
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        <span class="w3-xlarge">18,904,992 </span><br>
        Sequences
      </div>
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        <span class="w3-xlarge">115,650,683</span><br>
        Reads
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    <h2 class="w3-text-black">Cite this work</h2>
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    <p>First release:</p>
    <p>
      [1] Larriba, E., del Mazo, J. An integrative piRNA analysis of mouse gametes and zygotes reveals new potential origins and gene regulatory roles. Sci Rep 8, 12832 (2018). https://doi.org/10.1038/s41598-018-31032-1
    </p>

        <p>Present work:</p>
    <p>
        Paper in preparation
    </p>

	  <p>Image:</p>
    <p>
[2] Yamaguchi, S., Oe, A., Nishida, K.M. et al. Crystal structure of Drosophila Piwi. Nat Commun 11, 858 (2020).
https://doi.org/10.1038/s41467-020-14687-1
    </p>


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      <p><i class="fa fa-home  fa-fw w3-xxlarge w3-margin-right"></i> CIB Margarita Salas, CSIC</p>
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      <p><i class="fa fa-envelope fa-fw w3-xxlarge w3-margin-right"> </i> Email: odei.barrenada@cib.csic.es & jdelmazo@cib.csic.es </p>
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