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| 1 | + seqfile = sample.txt * sequence data filename |
| 2 | + treefile = samplephy.phy * tree structure file name |
| 3 | + outfile = mlc * main result file name |
| 4 | + |
| 5 | + noisy = 0 * 0,1,2,3,9: how much rubbish on the screen |
| 6 | + verbose = 0 * 0: concise; 1: detailed, 2: too much |
| 7 | + runmode = 0 * 0: user tree; 1: semi-automatic; 2: automatic |
| 8 | + * 3: StepwiseAddition; (4,5):PerturbationNNI; -2: pairwise |
| 9 | + |
| 10 | + seqtype = 1 * 1:codons; 2:AAs; 3:codons-->AAs |
| 11 | + CodonFreq = 0 * 0:1/61 each, 1:F1X4, 2:F3X4, 3:codon table |
| 12 | + clock = 0 * 0:no clock, 1:clock; 2:local clock; 3:CombinedAnalysis |
| 13 | + aaDist = 0 * 0:equal, +:geometric; -:linear, 1-6:G1974,Miyata,c,p,v,a |
| 14 | + aaRatefile = dat/jones.dat * only used for aa seqs with model=empirical(_F) |
| 15 | + * dayhoff.dat, jones.dat, wag.dat, mtmam.dat, or your own |
| 16 | + model = 0 |
| 17 | + * models for codons: |
| 18 | + * 0:one, 1:b, 2:2 or more dN/dS ratios for branches |
| 19 | + |
| 20 | + NSsites = 0 * 0:one w;1:neutral;2:selection; 3:discrete;4:freqs; |
| 21 | + * 5:gamma;6:2gamma;7:beta;8:beta&w;9:betaγ |
| 22 | + * 10:beta&gamma+1; 11:beta&normal>1; 12:0&2normal>1; |
| 23 | + * 13:3normal>0 |
| 24 | + |
| 25 | + icode = 0 * 0:universal code; 1:mammalian mt; 2-10:see below |
| 26 | + fix_kappa = 0 * 1: kappa fixed, 0: kappa to be estimated |
| 27 | + kappa = 3 * initial or fixed kappa |
| 28 | + fix_omega = 1 * 1: omega or omega_1 fixed, 0: estimate |
| 29 | + omega = 1 * initial or fixed omega, for codons or codon-based AAs |
| 30 | + |
| 31 | + fix_alpha = 1 * 0: estimate gamma shape parameter; 1: fix it at alpha |
| 32 | + alpha = 0 * initial or fixed alpha, 0:infinity (constant rate) |
| 33 | + Malpha = 0 * different alphas for genes |
| 34 | + ncatG = 8 * # of categories in dG of NSsites models |
| 35 | + |
| 36 | + getSE = 0 * 0: don't want them, 1: want S.E.s of estimates |
| 37 | + RateAncestor = 0 * (0,1,2): rates (alpha>0) or ancestral states (1 or 2) |
| 38 | + |
| 39 | + Small_Diff = .5e-6 |
| 40 | + cleandata = 1 * remove sites with ambiguity data (1:yes, 0:no)? |
| 41 | + method = 1 * Optimization method 0: simultaneous; 1: one branch a time |
| 42 | + |
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