1010 MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
1111 GNU General Public License for more details.
1212
13-
14- ##############################################################################
1513# AlignmentProcessor1.1 Package
1614
17- # Dependencies:
18- Python 3
19- Python 3 version of Biopython
20- PAML (if using CodeML)
21- PhyML (if using CodeML)
22- ##############################################################################
15+ # Dependencies:
16+ Python 3
17+ Python 3 version of Biopython
18+ PAML (if using CodeML)
19+ PhyML (if using CodeML)
20+
2321
2422AlignmentProcessor is a pipeline meant to quickly convert a multi-fasta
2523alignment file into a format that can be read by KaKs_Calculator or PAML and
@@ -31,15 +29,15 @@ based off of its sequences.
3129You can run the AlignmentProcessor wrapper which will call all of the python
3230scripts in sequence, or you may call each script individually.
3331
34- ### Installation
32+ # Installation
3533
36- # GitHub
34+ ### GitHub
3735AlignmentProcessor is freely available on GitHub and can either be downloaded directly from the site,
3836can cloned using the following command:
3937
4038 git clone https://github.com/WilsonSayresLab/AlignmentProcessor.git
4139
42- # Installing Biopython
40+ ### Installing Biopython
4341Since Biopython offers the fastest and easiest ways to deal with alignments
4442in a python script, it is used in several of the scripts in this package.
4543The easiest way to install Biopython is to download a Python 3 version
@@ -52,25 +50,25 @@ a terminal and Anaconda will install Biopython for you:
5250
5351 conda install -c https://conda.anaconda.org/anaconda biopython
5452
55- # KaKs_Calculator
53+ ### KaKs_Calculator
5654AlignmentProcessor0.21 is packaged with KaKs_Calculator2.0 binaries for Linux
5755and Windows, and a KaKs_Calculator1.2 binary for Mac (there is no 2.0 binary
5856available for OSX). Before using, copy or move the appropriate binary for your
5957system into the AlignmentProcessor bin which contains the python scripts.
6058
61- # PAML
59+ ### PAML
6260If you plan to use CodeML, you must first download PAML
6361(http://abacus.gene.ucl.ac.uk/software/paml.html ) and move the folder into the
6462AlignmentProcessor directory. Make sure that it is titled "paml".
6563
66- # PhyML
64+ ### PhyML
6765If you plan to use CodeML, you must also download PhyML
6866(http://www.atgc-montpellier.fr/phyml/binaries.php ). Similar to PAML, you must
6967move the folder into the AlignmentProcessor directory and change the name of
7068both the folder and the binary for your operating system to "PhyML".
7169
72- ### Quick Start
70+ # Quick Start
7371
7472 python AlignmentProcessor.py --axt/phylip --kaks/codeml -r <reference species> -i <input fasta file> -o <path to output directory>
7573
76- # Please see AlignmentPRocessorReadMe .pdf for specific options and instructions.
74+ ### Please see AlignmentProcessorReadMe .pdf for specific options and instructions.
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