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NGS_Preprocessing_Pipelines

Snakemake pipelines for preprocessing of NGS Illumina short read data including RNA-seq, Whole genome sequencing and exome sequencing data

Prerequisites

  • Software packages

  • Reference genomes

Software Installation

Running the NGS Pipelines

  • RNA-seq Pipeline

    run_snakemake.sh path/to/unaligned/ RNA genome_version paired_or_single_end subsample_option

  • Whole Genome Sequencing Analysis Pipeline

    run_snakemake.sh path/to/unaligned/ gDNA genome_version paired_or_single_end subsample_option

  • Exome-Seq Pipeline

    run_snakemake.sh path/to/unaligned/ Exome genome_version paired_or_single_end subsample_option

Contact

CCRSF_IFX@nih.gov