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> Seqsum was rewritten in Rust in 0.3.0. The original Python version of seqsum and how to use is archived in the [`python`](https://github.com/bede/seqsum/tree/python) branch. It remains available on PyPI.
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> Seqsum was rewritten in Rust in 0.3.0. The original Python version of seqsum and how to use it is archived in the [`python`](https://github.com/bede/seqsum/tree/python) branch. It remains available on PyPI.
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Robust checksums for nucleotide sequences. Accepts one or more `fast[a|q][.gz|.zst]` files or standard input. Generates an *aggregate* checksum for each input file by default, similar to `md5sum`/`sha256sum`. Warnings are shown for duplicate sequences and within-collection checksum collisions at the selected bit depth. Sequences are uppercased before hashing with [RapidHash](https://github.com/Nicoshev/rapidhash)(v3) and may be normalised (with `-n`) to use only `ACGTN-`. Read IDs and FASTQ base quality scores do not inform the checksum. Output is tab-delimited text to stdout.
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Robust checksums for nucleotide sequences. Accepts one or more `fast[a|q][.gz|.zst]` files or standard input. Generates an *aggregate* checksum for each input file by default, similar to `md5sum`/`sha256sum`. Warnings are shown for duplicate sequences and within-collection checksum collisions at the selected bit depth. Sequences are uppercased before hashing with [RapidHash](https://github.com/Nicoshev/rapidhash) and may be normalised (with `-n`) to use only `ACGTN-`. Read IDs and FASTQ base quality scores do not inform the checksum. Output is tab-delimited text to stdout.
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By default, seqsum outputs one aggregate checksum per file. Use `--individual` (`-i`) for per-record checksums, or `--all` (`-a`) for both individual and aggregate checksums. These flags are mutually exclusive.
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