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We use our OpenSPIM to study how early mammalian embryo models (using so-called mouse gastruloids) grow and develop, and how the first organs form. Especially, we are interested in how cells self-organize and orchestrate their motion to form tissue. To explain the underpinning dynamics, we couple our experiments with 3D simulations. To fully utilize light-sheet imaging, we also study collective cell migration and how nerve cells make new connections in live zebrafish, and we study the cells' own recycling system (autophagy) in Drosophila larvae. https://www.uio.no/ritmo/english/people/postdoctoral-fellows/endrejm/index/ - The illumination laser (Cobolt 6 series, Hübner Photonics) has laser heads with four different wavelengths (λ = 375, 488 561, 647 nm), corresponding to UV, green, red and far red light. - Fast imaging: An Arduino UNO board sends trigger pulses to the laser to obtain fast switching between the different wavelengths (ideal for live imaging). - Micro Manager controls the laser, camera (Andor Zyla 5.5, Andor), moveable 4D stage (USB-4D-Stage, Picard Industries), and the Arduino UNO board. - Temperature control: To control the temperature, water at physiological temperature flows through channels in the viewing chamber, which is made of heat conducting aluminum. - Media exchange: To provide fresh oxygen and nutrients to the 3D cell cultures, the media can be exchanged continually.
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Who_has_an_OpenSPIM.md

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# OsloSPIM at the University of Oslo, Norway
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We use our OpenSPIM to study how early mammalian embryo models (using so-called mouse gastruloids) grow and develop, and how the first organs form. Especially, we are interested in how cells self-organize and orchestrate their motion to form tissue. To explain the underpinning dynamics, we couple our experiments with 3D simulations. To fully utilize light-sheet imaging, we also study collective cell migration and how nerve cells make new connections in live zebrafish, and we study the cells' own recycling system (autophagy) in Drosophila larvae.
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https://www.uio.no/ritmo/english/people/postdoctoral-fellows/endrejm/index/
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- The illumination laser (Cobolt 6 series, Hübner Photonics) has laser heads with four different wavelengths (λ = 375, 488 561, 647 nm), corresponding to UV, green, red and far red light.
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- Fast imaging: An Arduino UNO board sends trigger pulses to the laser to obtain fast switching between the different wavelengths (ideal for live imaging).
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- Micro Manager controls the laser, camera (Andor Zyla 5.5, Andor), moveable 4D stage (USB-4D-Stage, Picard Industries), and the Arduino UNO board.
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- Temperature control: To control the temperature, water at physiological temperature flows through channels in the viewing chamber, which is made of heat conducting aluminum.
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- Media exchange: To provide fresh oxygen and nutrients to the 3D cell cultures, the media can be exchanged continually.
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