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Hormone Induced Spawning of Toads

Hormones

hCG

CAS 9002-61-3
Sigma C1063 or CG5

GnRH

CAS 79561-22-1
(Des-Gly¹⁰, D-Ala⁶, Pro-NHEt⁹)-LHRH acetate salt
Pyr-His-Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-NHEt acetate salt
Bachem H4070

Hormone Preparation

hCG

Prepare hCG solutions with a concentration of 2.5 IU per µL in Sterile Phosphate Buffered Saline

GnRH

Injection

Female

Priming Doses

Two priming doses given 72 hours apart hCG

Ovulatory Dose

Given 48 hours after the second priming dose

hCG

GnRH

Male

Inject and introduce to female 8-10 hours after female ovulatory dose

hCG

GnRH