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io_test.go
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package main
import (
"os"
"reflect"
"strings"
"testing"
"github.com/shenwei356/bio/seqio/fastx"
"github.com/shenwei356/xopen"
)
// Test header metric parsing
func TestParseHeaderMetrics(t *testing.T) {
tests := []struct {
name string
input string
want []HeaderMetric
wantErr bool
}{
{
name: "Empty string",
input: "",
want: nil,
wantErr: false,
},
{
name: "Valid metrics",
input: "avgphred,maxee,length",
want: []HeaderMetric{
{Name: "avgphred", IsLength: false},
{Name: "maxee", IsLength: false},
{Name: "length", IsLength: true},
},
wantErr: false,
},
{
name: "Invalid metric",
input: "avgphred,invalid,length",
want: nil,
wantErr: true,
},
{
name: "Multiple length metrics",
input: "length,avgphred,length",
want: []HeaderMetric{
{Name: "length", IsLength: true},
{Name: "avgphred", IsLength: false},
{Name: "length", IsLength: true},
},
wantErr: false,
},
{
name: "Whitespace handling",
input: " avgphred , maxee , length ",
want: []HeaderMetric{
{Name: "avgphred", IsLength: false},
{Name: "maxee", IsLength: false},
{Name: "length", IsLength: true},
},
wantErr: false,
},
{
name: "Mixed valid and invalid",
input: "avgphred,invalid1,maxee,invalid2",
want: nil,
wantErr: true,
},
}
for _, tt := range tests {
t.Run(tt.name, func(t *testing.T) {
got, err := parseHeaderMetrics(tt.input)
if (err != nil) != tt.wantErr {
t.Errorf("parseHeaderMetrics() error = %v, wantErr %v", err, tt.wantErr)
return
}
if !reflect.DeepEqual(got, tt.want) {
t.Errorf("parseHeaderMetrics() = %v, want %v", got, tt.want)
}
})
}
}
// Test record writing with quality filters
func TestWriteRecord(t *testing.T) {
tests := []struct {
name string
record *fastx.Record
quality float64
minQualFilter float64
maxQualFilter float64
headerMetrics []HeaderMetric
wantWrite bool
wantHeader string
}{
{
name: "Quality within bounds",
record: createTestRecord("test1", "ACGT", "IIII"),
quality: 30.0,
minQualFilter: 20.0,
maxQualFilter: 40.0,
headerMetrics: []HeaderMetric{
{Name: "avgphred", IsLength: false},
{Name: "length", IsLength: true},
},
wantWrite: true,
wantHeader: "test1 avgphred=40.000000 length=4",
},
{
name: "Quality below minimum",
record: createTestRecord("test2", "ACGT", "$$$$"),
quality: 10.0,
minQualFilter: 20.0,
maxQualFilter: 40.0,
wantWrite: false,
wantHeader: "",
},
{
name: "Quality above maximum",
record: createTestRecord("test3", "ACGT", "IIII"),
quality: 45.0,
minQualFilter: 20.0,
maxQualFilter: 40.0,
wantWrite: false,
wantHeader: "",
},
{
name: "No header metrics",
record: createTestRecord("test4", "ACGT", "IIII"),
quality: 30.0,
minQualFilter: 20.0,
maxQualFilter: 40.0,
headerMetrics: nil,
wantWrite: true,
wantHeader: "test4",
},
{
name: "Header with maxee metric",
record: createTestRecord("test5", "ACGT", "IIII"),
quality: 30.0,
minQualFilter: 20.0,
maxQualFilter: 40.0,
headerMetrics: []HeaderMetric{
{Name: "maxee", IsLength: false},
{Name: "length", IsLength: true},
},
wantWrite: true,
wantHeader: "test5 maxee=0.000400 length=4",
},
{
name: "Header with meep metric",
record: createTestRecord("test5", "ACGT", "IIII"),
quality: 30.0,
minQualFilter: 20.0,
maxQualFilter: 40.0,
headerMetrics: []HeaderMetric{
{Name: "meep", IsLength: false},
{Name: "length", IsLength: true},
},
wantWrite: true,
wantHeader: "test5 meep=0.010000 length=4",
},
{
name: "Header with lqpercent metric",
record: createTestRecord("test6", "ACGT", "II$$"),
quality: 30.0,
minQualFilter: 20.0,
maxQualFilter: 40.0,
headerMetrics: []HeaderMetric{
{Name: "lqpercent", IsLength: false},
{Name: "length", IsLength: true},
},
wantWrite: true,
wantHeader: "test6 lqpercent=50.000000 length=4",
},
}
for _, tt := range tests {
t.Run(tt.name, func(t *testing.T) {
// Create a temporary file for testing
tmpfile, err := os.CreateTemp("", "test*.fastq")
if err != nil {
t.Fatal(err)
}
defer os.Remove(tmpfile.Name())
defer tmpfile.Close()
// Create writer using the temp file
writer, err := xopen.Wopen(tmpfile.Name())
if err != nil {
t.Fatal(err)
}
defer writer.Close()
// Test writeRecord
got := writeRecord(writer, tt.record, tt.quality, tt.headerMetrics, AvgPhred, DEFAULT_MIN_PHRED, tt.minQualFilter, tt.maxQualFilter)
if got != tt.wantWrite {
t.Errorf("writeRecord() = %v, want %v", got, tt.wantWrite)
}
// If the record should be written, verify the header
if tt.wantWrite {
// Close the writer to ensure all data is written
writer.Close()
// Read the file content
content, err := os.ReadFile(tmpfile.Name())
if err != nil {
t.Fatal(err)
}
// Extract the header from the FASTQ format (first line)
lines := strings.Split(string(content), "\n")
if len(lines) > 0 {
gotHeader := strings.TrimPrefix(lines[0], "@")
if gotHeader != tt.wantHeader {
t.Errorf("Header = %q, want %q", gotHeader, tt.wantHeader)
}
}
}
})
}
}