compgenomicslab/demultiplex-ont-illumina
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### PIPELINE FOR DEMULTIPLEXING NANOPORE READS WITH ILLUMINA LIBRARIES ### ### CREATED BY CLAUDIA SANCHIS LOPEZ 2023 ### ##PIPELINE## # # 1. Get the whole index (uniq and common sequences) of illumina in nanopore reads (using blastn) # 2. Get uniq index, the indentifier of each index and its coordinates # 3. Calculating text similarity distance of each uniq index, and generate output matrix of each adaper # 4. Extract the best match index according to the text similarity # 5. Asign a sample if the best match index make sense with the real combination of indexes used in the wet lab (samples_index_captura_mcps082023 file) # # # #FILES: # #./trimmed_reads.00.fasta: the input fasta file are the trimmed reads of nanopore # #./out_dir/ shows all the intermediate files created for one fraction (00) of the total analysis as example of the outputs # #./demultiplex.sh: the pipeline calling all the python scripts in this folder. # #./samples_index_captura_mcps082023: the experimental design of the indexes for each sample